W.Va. State University gets “FISHy” with research workshop


W.Va. State University gets “FISHy” with research workshop

INSTITUTE, W.Va. -- West Virginia State University will hold a three-day, hands-on workshop on plant chromosome preparation and the Fluorescent in situ Hybridization (FISH) method, Oct. 11-13 in 207 Hamblin Hall on campus.

“State is the only university in the 1890 land-grant community with the capabilities to host such a workshop,” says Dr. Padma Nimmakayala, research scientist, referring to WVSU’s status as one of the nation’s 18 universities established under a congressional land-grant act in 1890.

FISH is a molecular technique used to identify specific plant, animal or microbe species. It can determine whether specific gene elements exist in a sample and if that gene is being expressed under a given set of conditions. Flourescent probes are attached to specific genetic regions of genomes to differentiate them from the other groups.

“After the probes are hybridized,” says Dr. Umesh K. Reddy, research scientist, “a fluorescent microscope is used to detect the presence or absence of individual signals.”

WVSU’s Agricultural and Environmental Research Station obtained an epi-fluorescent microscope system in 2010, when it began using the FISH method in the genomics lab of Reddy and Nimmakayala.

The workshop will include hands-on experience with the FISH method, as well as presentations and discussion regarding methodology and troubleshooting. There is no fee to attend, but registration is requested by contacting Reddy at 304-766-3258 or ureddy@wvstateu.edu. Students, faculty, and others interested in the FISH method are encouraged to attend.

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